Figure 1

Sequence-independent single-primer-amplification (SISPA) as a screening technique for detecting unexpected RNA viral adventitious agents in cell cultures

Behnam Alirezaie*, Davood Bahador, Ashraf Mohammadi and Abolhasan Foroughi

Published: 12 March, 2021 | Volume 5 - Issue 1 | Pages: 008-012

abb-aid1022-g001

Figure 1:

(A) Estimation of the detection limit of SISPA method using 10-fold serial dilution of the Challenge Virus Representing an Adventitious Agent. The mumps virus containing 106 CCID50/ml was serially diluted and each dilution subjected to method. (B and C) Agarose gel electrophoresis of PCR amplicons of the recombinant plasmids with universal M13 primers and the K primer (two of the several reactions are shown here). Lane MW is the 100 bp DNA lader marker (YTA, Iran).

Read Full Article HTML DOI: 10.29328/journal.abb.1001022 Cite this Article Read Full Article PDF

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